of cryoprotectants on the activity of selected embryonic enzymes in fish embryos

Abstract

Cryoprotectants, which are essential for minimizing cryoinjury during freezing, can be toxic to biological systems. Monohydric alcohols, dimethyl sulfoxide (Me2SO), and ethylene glycol (EG), are known to denature enzymes at room temperature. In this study, rosy barb (Puntius conchonius) and zebra fish (Brachydanio rerio) embryos at cleavage, epiboly, and closure of blastopore stages were exposed to Me2SO and EG at 1.0, 2.0, 3.0, and 4.0 M for 0.25. 0.5, 1.0, 2.0, and 3.0 at room temperature. The cryoprotectants were then removed and the activities of two glycolytic enzymes, lactate dehydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G-6-PDH), were determined